anti sod3  (Proteintech)

Journal: Journal of Molecular Neuroscience

Article Title: Proteomic Profiling and Therapeutic Targeting of Oxidative Stress in Autoimmune Encephalitis

doi: 10.1007/s12031-025-02332-9

Figure Lengend Snippet: Oxidative stress-related models for diagnosing patients with AE. A – C NaiveBayes, RandomForest, and SVM for selection of oxidative stress-related variables for differentiating patients with AE from controls. D – F ROCs of NaiveBayes, RandomForest, and SVM models in diagnosing patients with AE in training, test, and total sets. G Venn diagram of the key oxidative stress-related variables (ALB, APOE, GPX3, and SOD3) through intersecting the three models. H ROCs of ALB, APOE, GPX3, and SOD3 in diagnosing patients with AE. I Correlation analysis of ALB, APOE, GPX3, and SOD3 with oxidative stress score

Article Snippet: After blocking at room temperature for 1 h, incubation with primary antibodies was conducted at 4 °C overnight, with subsequent HRP-bound goat anti-mouse or anti-rabbit secondary antibodies (1:5000; ZB2305 or ZB2301; ZSGB-BIO) at room temperature for 1 h. The used primary antibodies against ALB (1:1000; 16,475–1-AP), APOE (1:1000; 18,254–1-AP), GPX3 (1:1000; 3947–1-AP), SOD3 (1:1000; 14,316–1-AP), and GAPDH (1:5000; 60,004–1-Ig) were purchased from Proteintech.

Techniques: Selection

Journal: Journal of Molecular Neuroscience

Article Title: Proteomic Profiling and Therapeutic Targeting of Oxidative Stress in Autoimmune Encephalitis

doi: 10.1007/s12031-025-02332-9

Figure Lengend Snippet: Antioxidant ALB, APOE, GPX3, and SOD3 are lowly expressed both in the serum of AE patients and CNS of EAE C57BL/6 mice. A – E Western blot of ALB, APOE, GPX3, and SOD3 in spinal cord tissues of control and EAE mice. F Transcriptional regulatory network. Triangles represent predicted transcription factors, and the circle represents the four target genes (red, high expression in autoimmune encephalitis, and green, low expression in autoimmune encephalitis). Dashed lines represent the predicted transcriptional regulatory relationships in the two databases, and solid lines represent the predicted transcriptional regulatory relationships in the three databases. G Quantification of the fractions of immune cells in patients with AE and controls. H – K Correlations between ALB, APOE, GPX3 and SOD3, and immune cells; **** p < 0.0001

Article Snippet: After blocking at room temperature for 1 h, incubation with primary antibodies was conducted at 4 °C overnight, with subsequent HRP-bound goat anti-mouse or anti-rabbit secondary antibodies (1:5000; ZB2305 or ZB2301; ZSGB-BIO) at room temperature for 1 h. The used primary antibodies against ALB (1:1000; 16,475–1-AP), APOE (1:1000; 18,254–1-AP), GPX3 (1:1000; 3947–1-AP), SOD3 (1:1000; 14,316–1-AP), and GAPDH (1:5000; 60,004–1-Ig) were purchased from Proteintech.

Techniques: Western Blot, Control, Expressing

Journal: Journal of Molecular Neuroscience

Article Title: Proteomic Profiling and Therapeutic Targeting of Oxidative Stress in Autoimmune Encephalitis

doi: 10.1007/s12031-025-02332-9

Figure Lengend Snippet: Anti-oxidative stress by NAC elevates the expression of antioxidant ALB, APOE, GPX3, and SOD3 in the CNS of EAE C57BL/6 mice. A – H Immunofluorescence of A and B ALB, C and D APOE, E and F GPX3, and G and H SOD3 in spinal cord tissues of control mice, EAE mice, and NAC-administrated EAE mice. Scale bars, 50 µm; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: After blocking at room temperature for 1 h, incubation with primary antibodies was conducted at 4 °C overnight, with subsequent HRP-bound goat anti-mouse or anti-rabbit secondary antibodies (1:5000; ZB2305 or ZB2301; ZSGB-BIO) at room temperature for 1 h. The used primary antibodies against ALB (1:1000; 16,475–1-AP), APOE (1:1000; 18,254–1-AP), GPX3 (1:1000; 3947–1-AP), SOD3 (1:1000; 14,316–1-AP), and GAPDH (1:5000; 60,004–1-Ig) were purchased from Proteintech.

Techniques: Expressing, Immunofluorescence, Control

Journal: Journal of Molecular Neuroscience

Article Title: Proteomic Profiling and Therapeutic Targeting of Oxidative Stress in Autoimmune Encephalitis

doi: 10.1007/s12031-025-02332-9

Figure Lengend Snippet: NAC treatment attenuates oxidative stress, tissue-resident CD4 + and CD8 + T cells and neuroinflammation in EAE SJL mice. A and B Clinical score of EAE severity and latency to fall of control, EAE, and NAC-treated EAE mice. C – E Detection of C MDA content, D SOD activity, and E GSH content in spinal cord tissues of the above mice. F – J Western blot of F and G ALB, F and H APOE, F and I GPX3, and F and J SOD3 in spinal cord tissues of the above mice. K – N Flow cytometry of the proportions of tissue-resident K and L CD4 + and M and N CD8. + T cells in spinal cord tissues of the above mice. O – Q ELISA of the levels of O IFN-γ, P TNF-α and Q IL-1β in spinal cord tissues of the above mice; *** p < 0.001; **** p < 0.0001

Article Snippet: After blocking at room temperature for 1 h, incubation with primary antibodies was conducted at 4 °C overnight, with subsequent HRP-bound goat anti-mouse or anti-rabbit secondary antibodies (1:5000; ZB2305 or ZB2301; ZSGB-BIO) at room temperature for 1 h. The used primary antibodies against ALB (1:1000; 16,475–1-AP), APOE (1:1000; 18,254–1-AP), GPX3 (1:1000; 3947–1-AP), SOD3 (1:1000; 14,316–1-AP), and GAPDH (1:5000; 60,004–1-Ig) were purchased from Proteintech.

Techniques: Control, Activity Assay, Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay